Methods in biotechnology
A.A. 2018/2019
Learning objectives
The goal is to acquire knowledge and operational competencies in genomics, functional genomics and applications to plant improvement and to molecular taxonomy.The course is subdivided in 3 modules. The goal is to acquire knowledge and operational competencies in genomics, functional genomics and applications to plant improvement and to molecular taxonomy
Expected learning outcomes
"The student will acquire competencies in generating and analyzing
data through the genomics and functional-genomics techniques.
The student will be able to plan experiments using genomic
sequencing, gene expression analyses, and their integration with
metabolomics and proteomics. The student will be able to apply
genomics approaches in molecular taxonomy, to characterize
agri-food matrices.The student will acquire competencies in generating and analyzing data through the genomics and functional-genomics techniques. The student will be able to plan experiments using genomic sequencing, gene expression analyses, and their integration with metabolomics and proteomics. The student will be able to understand and apply knowledge in molecular taxonomy"
data through the genomics and functional-genomics techniques.
The student will be able to plan experiments using genomic
sequencing, gene expression analyses, and their integration with
metabolomics and proteomics. The student will be able to apply
genomics approaches in molecular taxonomy, to characterize
agri-food matrices.The student will acquire competencies in generating and analyzing data through the genomics and functional-genomics techniques. The student will be able to plan experiments using genomic sequencing, gene expression analyses, and their integration with metabolomics and proteomics. The student will be able to understand and apply knowledge in molecular taxonomy"
Periodo: Primo semestre
Modalità di valutazione: Esame
Giudizio di valutazione: voto verbalizzato in trentesimi
Corso singolo
Questo insegnamento non può essere seguito come corso singolo. Puoi trovare gli insegnamenti disponibili consultando il catalogo corsi singoli.
Course syllabus and organization
Edizione unica
Responsabile
Periodo
Primo semestre
Genomics
Programma
1. Techniques and approaches in genome, exome and epigenome sequencing; 2. Strategies for plant genome analysis and assembly; 3. Plant genome structure, synteny and evolution; 4. Applications of genomics in plant breeding; 5. Basics in genome editing; 6. Workshops will be conducted on sequence analysis and alignments; design of experiments in plant genomics;
Informazioni sul programma
The student is offered the opportunity to take the exam in oral form. The oral exam will include the presentation (15 minutes) of a scientific paper (or papers) broadly spanning the entire program. Additional 15 minutes will be devoted to 3 questions concerning the entire program. Alternatively, the student can take an oral exam composed of 4 questions about each of the main areas of the program (for a total of 12 questions).
Propedeuticità
Genetics; Biochemistry; Microbiology; Statistics
Prerequisiti
Your capacity to attend and understand the course will be greatly improved if you'll master the following topics:1.Mendelian and formal genetics2.DNA and gene structure3.Recombination and its use in molecular mapping4.Molecular markers5.QTL and QTL-mapping6.Basics in nucleic acids sequencing7.Basics in plant breeding (breeding in autogamous and allogamous species; molecular markers applied to plant breeding);8.Basics in molecular biology (plasmids, cloning techniques and strategies, bacterial and eukaryotes transformation, restriction enzymes and their use in molecular biology, DNA labeling, nucleic acid analysis)9.Basics in statistics10.Basics in PC use
Materiale di riferimento
The slides (in English) will be timely provided during the course and will include explanatory text; additionally, papers and books abstracts will be posted on the Ariel website of the course. Suggested text books:-Basics on plant genomics are available in "Genetica e Genomica2 (Barcaccia e Falcinelli eds.).
Functional genomics
Programma
- Techniques and approaches in RNA analysis and modulation;- Strategies for genome modification;- Integration of high throughput /content techniques (omics) and data analysis;- Workshops will be conducted on gene targeting; RNA expression analysis and
Informazioni sul programma
The exam can be written and/or oral.
Propedeuticità
Genetics; Biochemistry; Microbiology; Statistics
Prerequisiti
Your capacity to attend and understand the course will be greatly improved if you'll master the following topics:Mendelian and formal geneticsDNA and gene structureRecombination and its use in molecular mappingMolecular markersQTL and QTL-mappingBasics in nucleic acids sequencingBasics in plant breeding (breeding in autogamous and allogamous species; molecular markers applied to plant breeding);Basics in molecular biology (plasmids, cloning techniques and strategies, bacterial and eukaryotes transformation, restriction enzymes and their use in molecular biology, DNA labeling, nucleic acid analysis)Basics in statisticsbasics in PC use
Materiale di riferimento
The slides (in English) will be timely provided during the course and will include explanatory text; additionally, papers and books abstracts will be posted on the Ariel website of the course.
Molecular taxonomy
Programma
1. Species concepts: which one we use in molecular taxonomy 2. Overview of taxonomic characters 3. Principles and methods of molecular taxonomy 4. Applications of molecular taxonomy from agri-food security to biodiversity and biomonitoring 5. Molecular markers 6. Reference databases and ad-hoc built databases 7. DNA barcoding and metabarcoding: from single organism to communities 8. Single marker vs multiple markers, PCR-free gene capture approaches 9. Workshops will be conducted on experimental design, primer selection and library preparation, basic skills on bash environments, analyses of NGS data and taxonomy assignment
Informazioni sul programma
Oral examination.The oral exam will include the presentation (15 minutes) of a scientific paper (or papers) broadly spanning the entire program. Additional 15 minutes will be devoted to 3 questions concerning the entire program. Alternatively, the student can take an oral exam composed of 4 questions about each of the main areas of the program (for a total of 12 questions).
Propedeuticità
Genetics; Biochemistry; Microbiology; Statistics
Prerequisiti
1. biology of the cell and organelles2. gene structure and function; from gene to function3. Basic molecular biology techniques (plasmids, cloning techniques and strategies, bacterial and eukaryotes transformation, restriction enzymes and their use in molecular biology, DNA labeling, nucleic acid analysis)4. Basics in statistics5. Basics in PC use
Materiale di riferimento
The slides (in English) will be timely provided during the course and will include explanatory text; additionally, papers and books abstracts will be posted on the Ariel website of the course. Suggested text books:-Molecular Plant Taxonomy - Methods and Protocols. Ed. Pascale Besse. Humana Press, 2014.-Environmental DNA for biodiversity research and monitoring. P. Taberlet, A. Bonin, L. Zinger, E. Coissac. Oxford University Press, 2018.
Moduli o unità didattiche
Functional genomics
AGR/12 - PATOLOGIA VEGETALE - CFU: 3.5
Esercitazioni: 16 ore
Laboratori: 8 ore
Lezioni: 16 ore
Laboratori: 8 ore
Lezioni: 16 ore
Docente:
Pasquali Matias
Genomics
AGR/07 - GENETICA AGRARIA - CFU: 3.5
Esercitazioni: 24 ore
Lezioni: 16 ore
Lezioni: 16 ore
Docente:
Pozzi Carlo Massimo
Molecular taxonomy
AGR/11 - ENTOMOLOGIA GENERALE E APPLICATA - CFU: 3
Laboratori: 28 ore
Lezioni: 10 ore
Lezioni: 10 ore
Docenti:
Gabrieli Paolo, Montagna Matteo
Professor(s)
Ricevimento:
Dal lunedì al venerdì su appuntamento o dopo lezione
Via Celoria 26 o in aula lezione
Ricevimento:
su appuntamento
R056 (via celoria 2)
Ricevimento:
Su appuntamento / upon request
Office - building 21010