Biomolecular Methodologies
A.Y. 2018/2019
Learning objectives
To understand the fundamentals of biochemical methodologies used in food analysis and characterization, also relevant to food quality and safety
Expected learning outcomes
At the end of this course, the student will acquire the following abilities: - to carry out a critical analysis and interpretation of the results obtained by the various biochemical methodologies for food characterization - to identify the most appropriate methods, criteria, and markers for defining ¿molecular quality indexes¿
Lesson period: Second semester
Assessment methods: Esame
Assessment result: voto verbalizzato in trentesimi
Single course
This course cannot be attended as a single course. Please check our list of single courses to find the ones available for enrolment.
Course syllabus and organization
Single session
Responsible
Lesson period
Second semester
Course syllabus
Formal classes (4 CFU) Fundamentals of the principal biochemical approaches for separation, identification and quantification of biological macromolecules, and their application in defining the quality of ingredients and/or in foods: - chromatography: gel filtration, ion exchange, hydrophobic and reverse phase chromatography - electrophoresis: SDS-PAGE, IEF, 2D electrophoresis - application of mass spectrometry to food issues - immunochemical and immunoenzymatic techniques: dot-blotting, Western blotting, and ELISA Spectroscopic approaches to study the structural modifications of macromolecules and their relevance to food quality and consumerism. Application of molecular biology approaches to food safety issues. Presentation and critical analysis of "case studies".
Teaching methods
Copies of any original visual aids are circulated among students. The following books, in Italian, are available for further reading: Campbell/Farrel, Biochimica, EdiSES; Duranti/Pagani, Metodologie di base per la biochimica e la biotecnologia, Zanichelli; De Marco/Cini, Principi di Metodologia Biochimica, Piccin.
BIO/10 - BIOCHEMISTRY - University credits: 4
Laboratories: 16 hours
Lessons: 24 hours
Lessons: 24 hours
Professor:
Iametti Stefania
Professor(s)