High-throughput screenings
A.A. 2022/2023
Learning objectives
Non definiti
Expected learning outcomes
Non definiti
Periodo: Primo semestre
Modalità di valutazione: Esame
Giudizio di valutazione: voto verbalizzato in trentesimi
Corso singolo
Questo insegnamento non può essere seguito come corso singolo. Puoi trovare gli insegnamenti disponibili consultando il catalogo corsi singoli.
Course syllabus and organization
Edizione unica
Responsabile
Programma
· The basis of High throughput screening:
o Target identification and Assay development
o Automation and miniaturization, liquid handling,
o Detection and data analysis
· Computational high throughput screening
· Biochemical High throughput screening
· Phenotypic screenings and cell-based high throughput screening
o High-content versus high-throughput
· In vivo based high throughput screening
· Small molecule-based screenings and drug discovery in academy vs an industrial environment
· Genetic high throughput screenings
o RNAi/shRNA-based screenings
o CRISPR-Cas9 based screenings
· New approaches:
o iPSC and whole organ modeling
o cancer-on-a-chip
o Target identification and Assay development
o Automation and miniaturization, liquid handling,
o Detection and data analysis
· Computational high throughput screening
· Biochemical High throughput screening
· Phenotypic screenings and cell-based high throughput screening
o High-content versus high-throughput
· In vivo based high throughput screening
· Small molecule-based screenings and drug discovery in academy vs an industrial environment
· Genetic high throughput screenings
o RNAi/shRNA-based screenings
o CRISPR-Cas9 based screenings
· New approaches:
o iPSC and whole organ modeling
o cancer-on-a-chip
Prerequisiti
Non sono richieste conoscenze preliminari.
Metodi didattici
Alle lezioni frontali saranno affiancate esercitazioni, lavori di gruppo e spazi di riflessione e discussione.
Materiale di riferimento
We will not use a main textbook, but provide the students with:
· All of the slides presented during the course
· PDF version of all of the articles/reviews presented during the course
· Web addresses of all of the relevant sites (presented during the course)
· All of the slides presented during the course
· PDF version of all of the articles/reviews presented during the course
· Web addresses of all of the relevant sites (presented during the course)
Modalità di verifica dell’apprendimento e criteri di valutazione
-Written test (50% of the evaluation), with 2 open questions regarding the topics presented in the course.
-During the course, the students will be divided in small teams (2-3) to prepare journal clubs which will be discussed with the teacher (50% of the evaluation).
Results will be communicated to the students individually by e-mail. The test can be discussed with the Course Supervisor, by appointment.
-During the course, the students will be divided in small teams (2-3) to prepare journal clubs which will be discussed with the teacher (50% of the evaluation).
Results will be communicated to the students individually by e-mail. The test can be discussed with the Course Supervisor, by appointment.
BIO/11 - BIOLOGIA MOLECOLARE
MED/04 - PATOLOGIA GENERALE
MED/04 - PATOLOGIA GENERALE
Esercitazioni: 16 ore
Lezioni: 40 ore
Lezioni: 40 ore
Professor(s)
Ricevimento:
Previo appuntamento da concordare via e-mail
Campus IEO, Via Adamello, 16 Milano