Development biology and differentiation
A.A. 2018/2019
Learning objectives
The identification of the effects of xenobiotics on development and tissue differentiation is a major branch of toxicology. The course will provide the students with current knowledge on the basis of the biological, molecular and genetic mechanisms that take place from gastrulation to organ formation and tissue differentiation. Particular emphasis will be given to studies in model organisms. Therefore, the course will focus on the developmental programs of mouse and zebrafish, and their application to study the effects of toxic agents and on state-of-the-art technologies to study the effect of xenobiotics in living animals (e.g. in vivo imaging) as well as in autoptic tissues and provide the basis for the understanding of the exploitation of these model organisms for small-scale and high-throughput analyses aimed at the quantitative and qualitative analysis of toxicant effects in living organisms.
Expected learning outcomes
Non definiti
Periodo: Primo semestre
Modalità di valutazione: Esame
Giudizio di valutazione: voto verbalizzato in trentesimi
Corso singolo
Questo insegnamento non può essere seguito come corso singolo. Puoi trovare gli insegnamenti disponibili consultando il catalogo corsi singoli.
Course syllabus and organization
Edizione unica
Responsabile
Periodo
Primo semestre
Programma
The course aims at giving the instruments necessary to understand the genetic, molecular and cellula rmechainsims involved in the development and differentiation of animals. The course will be divided in three parts
1. The first part will be devoted to understand the specific role of biological processes during development and differentiation (2CFU).
2. The second part will be devoted to understand the use of the different manipulations and techniques applied in the different animal models (1 CFU).
3. The third part will be devoted to understand the different phases of development and differentiation that lead to the formation of an adult organism (3 CFU).
- Experimental approaches and instruments used to study developmental biology.
- Cellular mechanisms involved in developmental biology: control of gene expression; cell-communications: the gradients and the induction; cell proliferation; cell death;
- Experimental animal models to study development:
C.Elegans, Drosophila Melanogaster, Danio Rerio, Mus Musculus
-Different phases of development and organogenesis: fertilisation, gastrulation, ectoderm, mesoderm and endoderm derivatives;
- The generation of transgenic, knockout animal models;
- The use of animal models to study the effect of toxic substances.
1. The first part will be devoted to understand the specific role of biological processes during development and differentiation (2CFU).
2. The second part will be devoted to understand the use of the different manipulations and techniques applied in the different animal models (1 CFU).
3. The third part will be devoted to understand the different phases of development and differentiation that lead to the formation of an adult organism (3 CFU).
- Experimental approaches and instruments used to study developmental biology.
- Cellular mechanisms involved in developmental biology: control of gene expression; cell-communications: the gradients and the induction; cell proliferation; cell death;
- Experimental animal models to study development:
C.Elegans, Drosophila Melanogaster, Danio Rerio, Mus Musculus
-Different phases of development and organogenesis: fertilisation, gastrulation, ectoderm, mesoderm and endoderm derivatives;
- The generation of transgenic, knockout animal models;
- The use of animal models to study the effect of toxic substances.
Prerequisiti
The course requires a basic knowledge in Biology. The examination consists in an oral presentation of an article covering topics of the lectures or a written exam on the topics of the course.
Materiale di riferimento
J.M.W Slack - Essential Developmental Biology
L. Wolpert - Principles of Development
L. Wolpert - Principles of Development
BIO/13 - BIOLOGIA APPLICATA - CFU: 6
Laboratory individual activity: 16 ore
Lectures: 40 ore
Lectures: 40 ore
Docente:
Cariboni Anna Maria
Professor(s)